The Niagara hives were inspected quickly again after the unexpected secondary split. The plastic hive was inspected, and a virgin queen was found again. The secondary split double NUC with unhatched queen cells was also inspected. I found a newly hatched virgin queen in that hive as well. The secondary split performed on Sunday, the day prior, was a success. We were able to catch the newly hatched queen before she killed off the unhatched cells. These cells were moved to a new box and those queens were able to hatch out.
In conclusion, our initial split of the plastic hive has now produced 3 colonies. One colony with a laying queen and two colonies with virgin queens.